Character Name : Host (Chapter 1: 'Wind') (voice)

Original Name : Amy Nicholson

Gender : Female

Character Name : Host (Chapter 2: 'Membranes') (voice)

Original Name : Rodney Ascher

Gender : Male

Character Name : Host (Chapter 3: 'Kindred') (voice)

Original Name : John Waters

Gender : Male

Character Name : Host (Chapter 4: 'Multitudes') (voice)

Original Name : Karyn Kusama

Gender : Female

Character Name : Host (Chapter 5: 'Judy') (voice)

Original Name : Justin Benson

Gender : Male

Character Name : Host (Chapter 5: 'Judy') (voice)

Original Name : Aaron Moorhead

Gender : Male

Character Name : Host (Chapter 6: 'Dig') (voice)

Original Name : David Lowery

Gender : Male

Character Name : Self (archive footage)

Original Name : David Lynch

Gender : Male

Character Name : Lounge Wizard (as Sid Pink)

Original Name : Jason Stoval

Gender : Male

Character Name : Self (archive footage)

Original Name : Judy Garland

Gender : Female

Character Name : Self (archive footage)

Original Name : Jay Leno

Gender : Male

Character Name : Self (archive footage)

Original Name : Jack Paar

Gender : Male